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Ellenberger and co-workers (eight) experienced intuited specifically this kind of motion of your OB area every time they solved the crystal structure from the much bigger human DNA ligase I (HuLig1; 919 amino acids) certain to an adenylylated nick. Their landmark composition was the very first to doc the circumferential envelopment of the DNA duplex by a ligase clamp and the ensuing bending and distortion in the duplex that drive the base pairs about the 3′-OH aspect of the nick into an RNA-like A helical conformation (eight). Equivalent distortions ended up observed during the ChVLig·DNA advanced (7).
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